ÓÐÄÄЩ¼ì²â·ÇÖÞÖíÎÁµÄÒÇÆ÷£¬·ÇÖÞÖíÎÁpcr¼ì²âÒÇ¡£·ÇÖÞÖíÎÁÊÇÒ»¼þ´«È¾ÐÔ¼«¶ÈÇ¿µÄÒß²¡£¬·ÇÖÞÖíÎÁÑϳÁµÄÓ°ÏìÁËÎÒ¹úÑøÖ³ÐÐÒµµÄ·¢Õ¹£¬²úÉúÁ˼«¶È´óµÄËðʧ¡£Òò¶øΪÁ˱£ÏÕÉúÖíÑøÖ³ÐÐÒµµÄ°²È«£¬Ô¤·À·ÇÖÞÖíÎÁµÄËðʧ½øÒ»²½À©´ó£¬Òò¶ø¾ÍÒª×öºÃ·ÇÖÞÖíÎÁµÄ·À¿Ø¹¤×÷¡£

ÔÚ·ÇÖÞÖíÎÁ·À¿Ø¹¤×÷ÖУ¬³ýÁË×öºÃÈÕ³£µÄÖÎÀí¡¢Ïû¶¾µÈ¹¤×÷£¬»¹Òª×öºÃ·ÇÖÞÖíÎÁµÄ¼ì²â¹¤×÷¡£ÔÚ·ÇÖÞÖíÎÁµÄ·À¿Ø¹¤×÷ÖÐҪʹÓüì²â·ÇÖÞÖíÎÁµÄÒÇÆ÷£¬ºÃ±È·ÇÖÞÖíÎÁpcr¼ì²âÒÇ¡£É½¶«CA88¹âµçÊÇרҵµÄ·ÇÖÞÖíÎÁpcr¼ì²âÒdzö²ú³§¼Ò£¬JD-PCR16·ÇÖÞÖíÎÁpcr¼ì²âÒǾÍÊÇÆäÖÐÒ»¿î£¬Æä¼¼Êõ²ÎÊýÈçÏÂ

ÑùÆ·ÈÝÁ¿£º16x0.2ml¡¢Ö§³Ö8Áª¹Ü

ºÏÓúIJģº³£¼ûͨÃ÷PCR ºÄ²Ä£¬8x0.2ml ÅŹÜ£¬0.2ml µ¥¹Ü

·´Ó³ÏµÍ³£º5-120ul·´Ó³Ä£Ê½ÏµÍ³

¼ÓÈÈ/ÔìÀäÄ£¿é£º½ø¿Ú°ëµ¼ÌåÈȵçÄ£¿é

ζȽÚÔìÁìÓò£º4¡ãC-99¡æ

ÆðÂäξùÔÈ¿ìÂÊ¡Ý2¡ãC/Ãë

οؾ«¶È£º¡Ü¡À0.1¡ãC

ζȾùÔÈÐÔ£º¡Ü¡À 0.2¡ãC

οØÇøÓòÊýÁ¿£º¶àµã£¨2 µã£©

ÌݶÈÊý£º0 ¸ö

ÌݶÈζÈÁìÓò£ºÎÞ

Ìݶȿ×Êý£ºÎÞ

Òý·¢¹âÔ´£ºÃâÊØ»¤led

Òý·¢¹â²¨³¤ÁìÓò£º400-700nm

¼ì²â²¿¼þ£º½ø¿Ú¹âµç¼ì²âÆ÷

¼ì²âͨ·Êý£º±êÅä 1ͨ·£¨FAM£©¡¢¸ßÅ䣨ѡÅ䣩£¨FAM¡¢VIC£©

ºÏÓÃȾÁϺÍ̽Õ룺FAM/SYBR Green I, VIC/HEX/CY3£¨Ñ¡Å䣩,  ROX/Texas Red(Ñ¡Åä), Cy5,TAMARA(Ñ¡Åä)

·ÇÖÞÖíÎÁpcr¼ì²âÒǵÄʹÓÿÉÄÜÔ®ÊÖÓû§¸üºÃµÄ×öºÃ·ÇÖÞÖíÎÁµÄ¼ì²â¹¤×÷£¬¶ÔÒß²¡×öµ½Ôç·¢ÏÖ¡¢ÔçÖÎÀí£¬½µµÍ·ÇÖÞÖíÎÁϰȾ·çÏÕ¡£

±¾ÎÄÍøÖ·£º/cjwt/1434.html